Two-photon microscopy
From Scholarpedia
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- Fritjof Helmchen*, Brain Research Institute, University of Zurich
- Winfried Denk, Max-Planck Institute for Medical Research, Heidelberg, Germany
Two-photon microscopy refers to a laser-scanning microscope technique that utilizes two-photon absorption processes for fluorescence excitation. Two-photon microscopy is especially well suited for high-resolution optical imaging relatively deep (<= 1 mm) in intact biological tissue.
